Objectives

  1. Immune suppression cancer
  2. Inflammation Cancer
  3. Cancer immune suppression
  4. Chemotherapy and immune activation by “Immunogenic tumour cell death”
  5. Immune Modulation Cancer vaccines and adoptive t cell therapy

Origins of cancer

The origin of cancers can be from a few places:

  • Genetic mutations on to oncogenes
  • Viral Concogenes or genome integration
  • Immune suppression leading to body being unable to clear cancer (transplant patients)
  • Chronic inflammation (sets up a nice microenvironment)

Cancer in transplant patients

Immunosuppressants suppress T cells and NK cells Incidence of viral and spontaneous cancers higher in immunosuppressed frouped as a result Solid cancers higher in transplant patients than in noraml population

Cancer arising from chronic inflammation

Increase collular proliferation leads to more chances of mutati. DNA damage from various sources like Reactive oxygen species released from neutrophils

Inflammation leads to cancer by altering the tumour microenvironment

Mechanisms are unclear but inflammation likely results in a pro metastatic niche

Infilaraiton of tumour promoting fibroblasts suppressive macrophages and myeloid derived suppressor cells

Immune suppression caused by cancer

Neoplastic tissue is a darwinian microenvironment (Balkwill et al,)

Tunmour cells must evase cell cycle control and the immune system, and therefore cancers are often made of the “fittest” tumour cells. Suppression can be local (in tumour) and systemic (often late stage)

Tumour antigens

Tumnours both overexpress self antigens and also produce new ones (as they are genomically unstable)

  • Neoantigens: tumour specific, result from mutations
  • Tumour associated antigens: result from dysregulation of gene expression

Cancer can be recognised by the immune system

Tumour can be rapidly eliminated or may exist over months or years Usual outcomes

  1. Elimination (tumour destroyed)
  2. Equilibrium (destruction of tumour cells with low fitness)
  3. Escape (tumour evades the immune system and grows)

How to target cancer using the immune system

Chemotherapy induced cancer call death and awakens the immune system to fight cancer. Dying tumour cells can be immunogenic

Usually cell death is quiet and immunosuppressant but radiation and chemotherapy induced severe cellular stress

Chemo can also inhibit myeloid derived suppressor cells (MDSC)

This can make tumour cells immunogenic and break the cycle of immune suppression

Cancer immunotherapy

  1. cancer vaccines
  2. checkpoint inhibitors
  3. Chimeric antigen receptors

An example of a vaccine that reduced cancer is the HPV vaccines

Immunising patients with tumour antigens but targeting viral agents of oncogenesis is

Adoptive T cell transfer

TIL

Culture T cells from inside tumours and give them hyping up chemicals reinject them also block molecules that c=induce T cell exhaustion (CTLA-4 and PD-1)

Slow to act be work even after drug withdrawal

Tumour specific CAR T cells into patients

Chimeric antigen receptor composed of an anticancer antigen antibody (B cell derived) and T cells signalling componenets

Patients T cells are transduced usinf retrovirus containing the CAR

Car is b cell derived and works well